Unlike other methods used to quantify mRNA e.g. Northern flashing and ribonucleaseic Protection analyses QPCR requires little RNA is less laborious and produces large amounts of data in a short time. If you have any questions please contact support using the contact information in the area that applies to you below. This parameter allows Primer3 to include the overlap of the primer with the destination range of the sequence as a term in the target function. Pan-viral Microarrays which contain representative sequences from all known viruses have been used to detect new and distant variants of known viruses. PCR targeting preserved genetic regions can be used to detect variants of known viruses but may not be able to detect any more divergent or completely new viruses as it is not a priori sequence data.
Unlike other methods used to quantify mRNA e.g. Northern blotting and ribonuclease protection assays qPCR requires little RNA is less labor intensive and produces large amounts of data in a short period of time.If you have any questions please contact customer care using the contact information in the region that applies to you below.This parameter allows Primer3 to include the overlap of the primer with the targeted area of the sequence as a term in the objective function.Pan-viral microarrays containing representative sequences from all known viruses have been used to detect novel and distantly-related variants of known viruses.PCR targeting conserved genetic regions can be used to detect variants of known viruses but may not be able to detect more divergent or completely novel viruses for which there exists no a priori sequence data.